Research Interests:
The Kolodner laboratory is studying the genetic and biochemical mechanisms of genetic recombination, DNA repair and suppression of spontaneous mutations primarily using Saccharomyces cerevisiae as a model system. Work in S. cerevisiae falls in two interrelated areas - 1) the analysis of the proteins and genes that function in DNA mismatch repair; and 2) elucidation of the pathways that prevent translocations and other types of gross chromosomal rearrangements, and the analysis of the proteins that function in these pathways. The Kolodner lab also has research interests in the area of investigating the genetics of cancer susceptibility and development that follows on previous studies showing that a common cancer susceptibility syndrome, Lynch Syndrome (hereditary non-polyposis colorectal cancer), is due to inherited defects in DNA mismatch repair genes. This work is focused on understanding whether genes that prevent genome instability act as tumor suppressor genes in mice and humans.
Track(s):
Genetics
MCB
BMS Focus Areas:
Cancer Biology
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Selected Publications
Wang, Y, Putnam, CD, Kane, MF, Zhang, W, Edelmann, L, Russel, R, Carrington, DV, Chin, L, Kucherlapati, R, Kolodner, RD, and Edelmann, W. Mutation in Rpa1 results in defective DNA double-strand break repair, chromosomal instability and cancer in mice. Nature Genet. 2005;37:750-755.
Mazur, DJ, Mendillo, ML, and Kolodner, RD. Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2(ATP)-Msh6(ATP) state that is capable of hydrolysis independent movement along DNA. Mol. Cell. 2006;22:39-49.
Enserink, JM, Smolka, MB, Zhou, H, and Kolodner, RD. Checkpoint proteins control morphogenetic events during DNA replication stress in Saccharomyces cerevisiae. J. Cell Biol. 2006;175:729-741.
Shell, SS, Putnam, CD, and Kolodner, RD. The N-terminus of Saccharomyces cerevisiae Msh6 is an unstructured tether to PCNA. Mol. Cell. 2007;26:565-578.
Putnam, CD, Hayes, TK, and Kolodner, RD. Specific pathways prevent duplication-mediated genome rearrangements. Nature (Article). 2009;460:984-989.
Pennaneach, V, and Kolodner, RD. Stabilization of dicentric translocations through secondary rearrangements mediated by multiple mechanisms in S. cerevisiae. PLoS One. 2009;4:e6389.
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