Photo of Theodore Friedmann

Theodore Friedmann

Professor of Pediatrics
M.D., University of Pennsylvania
Email: tfriedmann@ucsd.edu

Research Interests:

The historical interest of my laboratory has been in gene therapy and he development of efficient gene transfer methods. Our principal clinical model for gene therapy is the neurodevelopmental disorder Lesch Nyhan Disease caused by deficiency of the housekeeping HPRT gene and characterized by retardation, dystonia and self-mutilatory behavior. We have shown that HPRT deficiency dysregulates a number of vital signaling pathways and leads to dysregulation of neurogenesis and aberrant expression of genes required for faithful development of dopamine pathways and dopaminergic neurons. Our current studies involve gene expression studies in cell culture models and in a knockout mouse model of the disease. We are currently concentrating on characterizing the aberrant neurogenic mechanisms in human ES and iPS cells deficient in HPRT. Our general technology includes gene expression profiling, ES and iPS cell differentiation, ChIP-Sseq analysis of wild type and HPRT knockdown cells and micro-RNA dysregulation of neural pathways in HPRT deficiency. Another set of studies deals with the development of molecular signals of administration of doping agents in Sport and the use of computational methods for meta analysis of disparate datasets.

Track(s)

Genetics

Molecular Pathology

BMS Focus Areas:

Neurobiology

Stem Cells

Selected Publications:

 

1. Preparation of pseudotyped lentiviral vectors resistant to inactivation by serum complement.

Guibinga GH, Friedmann T. Cold Spring Harb Protoc. 2010 Jul 1;2010:pdb.prot5420. doi: 10.1101/pdb.prot5420.

 

2. Ethics. Gene doping and sport.

Friedmann T, Rabin O, Frankel MS. Science. 2010 Feb 5;327(5966):647-8. No abstract available.

 

3.Deficiency of the housekeeping gene hypoxanthine-guanine phosphoribosyltransferase (HPRT) dysregulates neurogenesis.

Guibinga GH, Hsu S, Friedmann T. Mol Ther. 2010 Jan;18(1):54-62. Epub 2009 Aug 11.

 

4.A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts.

King CC, Bouic K, Friedmann T. Proteome Sci. 2009 Aug 11;7:28.

 

5. Insulin-like growth factor-1 coordinately induces the expression of fatty acid and cholesterol biosynthetic genes in murine C2C12 myoblasts.

Bhasker CR, Friedmann T. BMC Genomics. 2008 Nov 11;9:535.

 

6.Characterization of the gene delivery properties of baculoviral-based virosomal vectors.

Guibinga GH, Song S, Loring J, Friedmann T. J Virol Methods. 2008 Mar;148(1-2):277-82. Epub 2008 Jan 18.

 

7.Partial characterization of the proteome of the mouse striatum.

Guo LT, Friedmann T, King CC. Proteomics. 2007 Nov;7(21):3867-9.

 

8.Tissue-specific aberrations of gene expression in HPRT-deficient mice: functional complexity in a monogenic disease?

Song S, Friedmann T. Mol Ther. 2007 Aug;15(8):1432-43.

9.Lessons for the stem cell discourse from the gene therapy experience. Friedmann T. Perspect Biol Med. 2005 Autumn;48(4):585-91.

PMID: 16227669 [PubMed - indexed for MEDLINE]