BIOM 230, FALL 2008, SCHEDULE

Dr. Tracy Handel

07/16/08

Course Directors

 

Structural And Quantitative Pharmacology

 

Class Times: Tues. Thurs, 1:00-2:30 p.m.; Friday, 1:00–3:00 p.m (as needed for labs, presentations, other)
Place: PSB, Education Center Room 2

Text: Handouts and Assigned Reading By Lecturers

 

Week

Date

Topic

Reading

Lecturer

1

 

Th 9/25

Intro to Course

Review Principles of Protein Structure

 

Handel

 

Tu 9/26

NMR

 

Handel

2

 

Tu 9/30

X-ray Crystallography

 

TBD

Th 10/2

Electron Microscopy/CryoEM

 

 

Dorit Hanein

 

Fri 10/3

Visualization and Analysis Tools I

Web-based

 

PeterRose/Andreas

(Bourne)

3

 

Tu 10/7

Protein Thermodynamics and Stability I

 

 

R. Abagyan

Th 10/9

Protein Thermodynamics and Stability II

 

 

R. Abagyan

Fri 10/10

Molecular Modeling/Molsoft ICM Visualization and Analysis Tools II

 

 

 

 

R. Abagyan

4

 

Tu 10/14

Enzyme Kinetics I:

Catalysis

 

 

J. Adams

Th 10/16

Enzyme Kinetics II:

Catalysis

 

 

J. Adams

Fri 10/17

PreSteady State and Rapid Kinetics

 

Dmitri Davydov

5

 

Tu 10/21

Protein-Protein Interactions with Focus on Fluorescence/FRET

 

Dmitri Davydov

Th 10/23

Mathematical Methods of Spectral Analysis

 

Dmitri Davydov

Fri 10/24

Receptor:Ligand Binding I

 

Handel

 

 

Week

Date

Topic

Reading

Lecturer

6

Tu 10/28

Receptor:Ligand Binding II

 

 

Handel

 

Th 10/30

Mass Spectrometry I

 

Pieter Dorrestein

Fri 10/31

Mass Spectrometry II

 

Pieter Dorrestein

7

Tu 11/4

Structure selection criteria and tools for selection and analysis

 

PeterRose/Andreas

(Bourne)

Th 11/6

Off-target Binding

 

 

Bourne

Fri 11/7

TBD or No class

 

 

8

Tu 11/11

Holiday

 

 

 

Th 11/13

Docking Lab/Drug Receptor Interactions (1-3pm)

 

Michael/Bourne

 

Fri 11/14

Small Angle X-ray Scattering and Bioinformatic Analysis of Complexes

 

Susan Taylor

 

9

 

Tu 11/18

GPCRs Structure and Activation Mechanisms

 

Student

Presentations

Th 11/20

G proteins

 

 

Student

Presentations

Fri 11/21

Protein Kinase Structure and Activation

 

 

Student

Presentations

10

 

Tu 11/25

Phosphatases

 

Student

Presentations

Th 11/27

Holiday

 

 

Fri 11/28

Holiday

 

 

 

11

 

Tu 12/2

In Vivo Studies of Signaling Complexes

 

Student

Presentations

Th 12/4

Ligand Gated Ion Channels

 

Student

Presentations

Fri 12/05

Voltage Gated Ion Channels

 

 

Student

Presentations

 

Long versions

Biom230, Structural and Quantitative Pharmacology:

The goal of this course is to give students a foundation in more quantitative approaches to studying topics in pharmacology. The first 2/3 of the course will be didactic and focused on topics related to protein structure, thermodynamic stability, energetics and interactions.  Lectures will fall into three general classes:  theoretical (structure, forces that stabilize proteins, thermodynamic stability, receptor-ligand binding theory, enzyme kinetics), methods (crystallography, NMR, mass spectrometry, fluorescence), and practical approaches (tools for visualization of structures, protein-ligand docking,  mathematical analysis of data). The last part of the course will be student driven with presentations on various topics in pharmacology that utilize quantitative concepts covered in the first part of the course, and present the current state of knowledge as well as future directions and questions. Examples include structure and activation mechanisms of GPCRs, G proteins, and Ion Channels. Grades will be based on the presentation and a short research proposal related to the presentation, as well as student participation throughout the course.

 

40 Word Limit Version

This course is essentially biophysical pharmacology. 2/3 will be didactic, covering: protein structure, thermodynamic stability, receptor-ligand binding, enzyme kinetics, biophysical methods, visualization of structures/docking, mathematical analysis of data. The last section will involve student presentations of topics in biophysical pharmacology.